Background: The Australian CRC for Molecular Plant Breeding (MPBCRC) has generated a resource of promoters for use in cereals and other crops. Examples of their expression specificities/prevalences include pollen-specific, vascular-tissue/xylem-associated, vascular-tissue/phloem-associated, leaf prevalent, stem-prevalent, defense response-associated and pericarp-specific. Chimeric promoter::reporter gene fusions (e.g., gusA and gfp) have been generated and evaluated in transgenic tobacco, rice and/or barley plants.

Having such a resource of promoters in the public domain would enable more effective development of a range of transgenic wheat varieties with improved traits such as disease and abiotic stress tolerance.

Objectives:

1. Transform each of the chimeric promoter::Reporter gene constructs into the highly-transformable MBP Bobwhite-26 wheat variety
2. Evaluate the level, tissue specificity and/or inducibility to each
3. Develop constructs that facilitate the combination of target genes with the most effective promoters

Target Germplasm: MPB Bobwhite-26

Gene(s): The reporter genes gusA and gfp

Partner(s): Department of Primary Industries (DPI), Australia

Status: Initial transformants are being produced.

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