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Background: A number of
genes have been isolated that are expressed early in embryo
development. As a component of developing an apomictic gene
system, it is necessary to identify promoters that produce
gene expression at this phase of development. We are evaluating
one such promoter from the Arabidopsis thaliana DMC1 gene
in maize.
Objective:
- Identify the effect and role of DMC1
promoter in meiosis and further development of maize inflorescences
Target Germplasm: CML216/CML72
maize hybrid
Gene(s): AtDMC1 (Disruption
Meiosis cDNA, eukaryotic equivalent of recA) from Arabidopsis
thaliana, provided by Dr. Andreas Betzner (Australian National
University, Canberra, Australia). GFP (Green Fluorescent Protein),
provided by Dr. Alfredo Herrera-Estrella (CINVESTAV-Irapuato).
Partner(s): Instituto
Nacional de Investigaciones Forestales, Agricolas y Pecuarias
(INIFAP), with funding from CONACYT.
Status: Transformants produced
and are being evaluated in CIMMYT’s Biosafety Greenhouse.
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